The Nuts and Bolts of Lineage Chimerism Testing
Chimerism analysis is used to monitor the outcome of allogeneic hematopoietic stem cell transplantation. By
looking at the presence of donor leukocytes within specific cell subsets of the host’s peripheral blood, one can assess the
success of donor cell engraftment or detect early graft failure, rejection, or relapse. Prior to performing chimerism analysis, different cell subsets must be isolated from the same sample to perform lineage-specific analysis. In this webinar, Dr.
Cathi Murphey (Immunogenetics Lab Director; Southwest Immunodiagnostics, Texas) will discuss available technologies for isolating different cell subsets from the same sample and improving cell purity prior to chimerism analysis.
Anticipating Cytopenia: In Vitro Methods for Hematotoxicity Testing
The screening of novel compounds to predict potential toxicity to the hematopoietic system (i.e., hematotoxicity) is an important step in drug development. This webinar discusses the importance of hematotoxicity testing in drug development, reviews methods for in vitro assay standardization, and explores the use of both colony-forming unit (CFU) assays and lineage-specific liquid culture-based assays as tools for in vitro drug screening.
Passaging Naive PSC Colonies Derived and Maintained in RSeT™
How to passage naive colonies derived in RSeT™ without transferring irradiated mouse embryonic fibroblasts (iMEFs) to the next passage, and what you can expect during the early stages of reversion