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EasySep™ HLA嵌合全血CD3正选试剂盒

从全血和白膜层中免疫磁珠正选人CD3+细胞
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产品号 #（选择产品）

产品号 #17871_C

从全血和白膜层中免疫磁珠正选人CD3+细胞

产品优势

  • 快速、易于操作
  • 分选纯度高达99%
  • 无需分离柱
  • 兼容 EasySep™、“The Big Easy”和RoboSep™-S平台

产品组分包括

  • EasySep™ HLA嵌合全血CD3正选试剂盒（产品号#17871）          
    • EasySep™ HLA嵌合全血CD3正选抗体混合物，3 X 1mL
    • EasySep™ Dextran RapidSpheres™ 50101 磁珠，3 X 1mL
    • EasySep™ 红细胞裂解液10倍浓缩液，10 mL（产品号#20110）
  • RoboSep™ HLA嵌合全血CD3正选试剂盒（含滤芯吸头）（产品号#17871RF）
    • EasySep™ HLA嵌合全血CD3正选抗体混合物，3 X 1mL
    • EasySep™ Dextran RapidSpheres™ 50101 磁珠，3 X 1mL
    • EasySep™ 红细胞裂解液10倍浓缩液，10 mL（产品号#20110）
    • RoboSep™ 缓冲液（产品号 #20104）x 2
    • RoboSep™过滤吸头（产品号#20125）x 2
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  1. The Big Easy EasySep™ Magnet
    "The Big Easy" EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
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    Cell separation buffer

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用EasySep™ HLA嵌合全血CD3正选试剂盒，通过免疫磁珠正选技术从新鲜全血或白膜层样本中分离高纯度人CD3+细胞。EasySep™技术结合单克隆抗体的特异性和无需分离柱的简便磁分选系统，已在发表的研究中广泛应用超过20年。

在该EasySep™正选流程中，目的细胞通过识别CD3的抗体复合物与磁珠进行标记。抗体复合物中还含有人Fc受体抗体，可最大程度地减少非特异性结合。使用EasySep™磁分选系统分离标记细胞，只需倾倒或移液吸取非目的细胞。目的细胞保留在分离管中。细胞分选后，目的CD3+细胞即可用于流式细胞术、培养或DNA/RNA提取等下游应用。

本产品替代EasySep™人全血CD3正选试剂盒（产品号#18081）和EasySep™ HLA全血CD3正选试剂盒（产品号#18081HLA），可实现更快速的细胞分选。

深入了解免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化以节省时间并提升实验室通量。探索更多为您的实验流程优化的产品，包括细胞表征、冷冻保存等相关产品。

在该EasySep™正选流程中，目的细胞通过识别CD3的抗体复合物与磁珠进行标记。抗体复合物中还含有人Fc受体抗体，可最大程度地减少非特异性结合。使用EasySep™磁分选系统分离标记细胞，只需倾倒或移液吸取非目的细胞。目的细胞保留在分离管中。细胞分选后，目的CD3+细胞即可用于流式细胞术、培养或DNA/RNA提取等下游应用。

本产品替代EasySep™人全血CD3正选试剂盒（产品号#18081）和EasySep™ HLA全血CD3正选试剂盒（产品号#18081HLA），可实现更快速的细胞分选。

深入了解免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化以节省时间并提升实验室通量。探索更多为您的实验流程优化的产品，包括细胞表征、冷冻保存等相关产品。

在该EasySep™正选流程中，目的细胞通过识别CD3的抗体复合物与磁珠进行标记。抗体复合物中还含有人Fc受体抗体，可最大程度地减少非特异性结合。使用EasySep™磁分选系统分离标记细胞，只需倾倒或移液吸取非目的细胞。目的细胞保留在分离管中。细胞分选后，目的CD3+细胞即可用于流式细胞术、培养或DNA/RNA提取等下游应用。

本产品替代EasySep™人全血CD3正选试剂盒（产品号#18081）和EasySep™ HLA全血CD3正选试剂盒（产品号#18081HLA），可实现更快速的细胞分选。

深入了解免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化以节省时间并提升实验室通量。探索更多为您的实验流程优化的产品，包括细胞表征、冷冻保存等相关产品。

在该EasySep™正选流程中，目的细胞通过识别CD3的抗体复合物与磁珠进行标记。抗体复合物中还含有人Fc受体抗体，可最大程度地减少非特异性结合。使用EasySep™磁分选系统分离标记细胞，只需倾倒或移液吸取非目的细胞。目的细胞保留在分离管中。细胞分选后，目的CD3+细胞即可用于流式细胞术、培养或DNA/RNA提取等下游应用。

本产品替代EasySep™人全血CD3正选试剂盒（产品号#18081）和EasySep™ HLA全血CD3正选试剂盒（产品号#18081HLA），可实现更快速的细胞分选。

深入了解免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化以节省时间并提升实验室通量。探索更多为您的实验流程优化的产品，包括细胞表征、冷冻保存等相关产品。

磁体兼容性
• “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)  
 
亚型
细胞分选试剂盒
 
细胞类型
T 细胞
 
种属
人
 
样本来源
Buffy Coat，Whole Blood
 
筛选方法
Positive
 
品牌
EasySep
 
研究领域
HLA，免疫
 

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实验数据

Typical EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Profile

Figure 1. Typical EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Profile

Starting with human whole blood, the CD3+ cell content of the isolated fraction typically ranges from 92.4 - 99.8% (as assessed by staining the start and isolated fractions with anti-CD3 or anti-CD2 antibodies, respectively). In the above example, the purities of the start and the final isolated fractions are 18.2% and 98.1%, respectively (gated on CD45).
NOTE: Red blood cells were removed from the start sample by lysis prior to flow cytometry.

FACS Data for Anti-Human CD2 Antibody, Clone RPA-2.10, PE-Conjugated

Figure 2. FACS Data for Anti-Human CD2 Antibody, Clone RPA-2.10, PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, PE (Catalog #60007PE) and anti-human CD45 APC. (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ HLA CD3 Positive Selection Kit (Catalog #17871) and labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (open histogram).

FACS Data for Anti-Human CD5 Antibody, Clone UCHT2, PE-Conjugated

Figure 3. FACS Data for Anti-Human CD5 Antibody, Clone UCHT2, PE-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD5 Antibody, Clone UCHT2, PE (Catalog #60082PE) and Anti-Human CD20 Antibody, Clone 2H7, APC (Catalog #60008AZ). (B) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa PE isotype control antibody and Anti-Human CD20 Antibody, Clone 2H7, APC. (C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA CD3 Positive Selection Kit (Catalog #17871) and labeled with Anti-Human CD5 Antibody, Clone UCHT2, PE. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Buffy Coat or Whole Blood
Product Name
RoboSep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit
Catalog #
17871RF
Lot #
1000119933 or higher
Language
English
Document Type
Product Information Sheet - Leukoreduction System Chamber/Cone
Product Name
RoboSep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit
Catalog #
17871RF
Lot #
1000119933 or higher
Language
English
Document Type
Product Information Sheet - Leukoreduction System Chamber/Cone
Product Name
EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit
Catalog #
17871
Lot #
1000119933 or higher
Language
English
Document Type
Product Information Sheet Buffy Coat or Whole Blood
Product Name
EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit
Catalog #
17871
Lot #
1000119933 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit
Catalog #
17871RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit
Catalog #
17871RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit
Catalog #
17871RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit
Catalog #
17871RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit
Catalog #
17871
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit
Catalog #
17871
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit
Catalog #
17871
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (14)

Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Technical Bulletin
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
4:55
Video
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Prepare a Buffy Coat from Whole Blood
3:46
Video
How to Prepare a Buffy Coat from Whole Blood
Exploring the Vitality of Lineage-Specific Chimerism Analysis After HSCT
18:50
Webinar
Exploring the Vitality of Lineage-Specific Chimerism Analysis After HSCT
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
56:02
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
The Nuts and Bolts of Lineage Chimerism Testing
33:41
Webinar
The Nuts and Bolts of Lineage Chimerism Testing
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subsets in as Little as 15 Minutes for Use in Lineage-Specific Chimerism Analysis
Scientific Poster
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subse...

文献 (1)

Neutrophil and Granulocytic Myeloid-Derived Suppressor Cell-Mediated T Cell Suppression Significantly Contributes to Immune Dysregulation in Common Variable Immunodeficiency Disorders. M. Vlkova et al. Journal of immunology (Baltimore, Md. : 1950) 2018 NOV

Abstract

Common variable immunodeficiency disorders (CVID) represent a group of primary immunodeficiency diseases characterized by hypogammaglobulinemia and impaired specific Ab response, resulting in recurrent infections due to dysfunctional immune response. The specific mechanisms mediating immune deficiency in CVID remain to be determined. Previous studies indicated that immune dysregulation in CVID patients is associated with chronic microbial translocation, systemic immune activation, and altered homeostasis of lymphocytic and myeloid lineages. A detailed phenotypic, functional characterization of plasma markers and immune cell populations was performed in 46 CVID patients and 44 healthy donors. CVID patients displayed significantly elevated plasma levels of a marker of neutrophil activation neutrophil gelatinase-associated lipocalin. Neutrophils from CVID patients exhibited elevated surface levels of CD11b and PD-L1 and decreased levels of CD62L, CD16, and CD80, consistent with a phenotype of activated neutrophils with suppressive properties. Neutrophils from CVID patients actively suppressed T cell activation and release of IFN-$\gamma$ via the production of reactive oxygen species. Furthermore, CVID was associated with an increased frequency of low-density neutrophils (LDNs)/granulocytic myeloid-derived suppressor cells. LDN/granulocytic myeloid-derived suppressor cell frequency in CVID patients correlated with reduced T cell responsiveness. Exogenous stimulation of whole blood with bacterial LPS emulated some but not all of the phenotypic changes observed on neutrophils from CVID patients and induced neutrophil population with LDN phenotype. The presented data demonstrate that neutrophils in the blood of CVID patients acquire an activated phenotype and exert potent T cell suppressive activity. Specific targeting of myeloid cell-derived suppressor activity represents a novel potential therapeutic strategy for CVID.
View publication
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更多信息

更多信息
种属 Human
Magnet Compatibility • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源 Buffy Coat, Whole Blood
Selection Method Positive
标记抗体
  1. 抗人CD20抗体，克隆2H7
    抗人CD20抗体，克隆2H7

    小鼠单克隆IgG2b抗体，抗人、恒河猴、食蟹猴CD20

  2. 抗人CD5 抗体，克隆UCHT2
    抗人CD5 抗体，克隆UCHT2

    小鼠单克隆IgG1抗体，抗人、恒河猴、食蟹猴CD5

  3. 山羊抗小鼠 IgG (H+L) 抗体，多克隆
    山羊抗小鼠 IgG (H+L) 抗体，多克隆

    抗小鼠IgG（H+L）山羊多克隆抗体

  4. 抗人CD2抗体，克隆RPA-2.10
    抗人CD2抗体，克隆RPA-2.10

    小鼠抗人、恒河猴、食蟹猴CD2的单克隆IgG1抗体

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