EasySep™ Human Neutrophil Isolation Kit

总览

使用EasySep™人中性粒细胞分离试剂盒，可轻松高效地从新鲜人外周血白细胞样本中通过免疫磁珠负选获得高纯度人中性粒细胞。EasySep™技术结合单克隆抗体的特异性和无柱磁分选系统的简便性，已在发表的研究中广泛应用超过20年。

在该EasySep™负选流程中，非目标细胞被抗体复合物与磁珠标记。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的中性粒细胞分离，接着简单地将目的细胞倾倒或吸取至一个新的试管中。磁珠分选最快仅需14分钟，目标中性粒细胞即可用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。本分离试剂盒兼容经HetaSep™（产品号 #07906）沉降法或红细胞裂解法处理的细胞样本。

该产品可替代EasySep™人中性粒细胞富集试剂盒 (产品号 #19257) 以进行更快地细胞分选。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。亦可选择即用型、符合伦理来源的人外周血中性粒细胞冻存产品。探索更多为您实验流程优化的其它产品，包括培养基、补充剂、抗体等。

在该EasySep™负选流程中，非目标细胞被抗体复合物与磁珠标记。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的中性粒细胞分离，接着简单地将目的细胞倾倒或吸取至一个新的试管中。磁珠分选最快仅需14分钟，目标中性粒细胞即可用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。本分离试剂盒兼容经HetaSep™（产品号 #07906）沉降法或红细胞裂解法处理的细胞样本。

该产品可替代EasySep™人中性粒细胞富集试剂盒 (产品号 #19257) 以进行更快地细胞分选。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。亦可选择即用型、符合伦理来源的人外周血中性粒细胞冻存产品。探索更多为您实验流程优化的其它产品，包括培养基、补充剂、抗体等。

在该EasySep™负选流程中，非目标细胞被抗体复合物与磁珠标记。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的中性粒细胞分离，接着简单地将目的细胞倾倒或吸取至一个新的试管中。磁珠分选最快仅需14分钟，目标中性粒细胞即可用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。本分离试剂盒兼容经HetaSep™（产品号 #07906）沉降法或红细胞裂解法处理的细胞样本。

该产品可替代EasySep™人中性粒细胞富集试剂盒 (产品号 #19257) 以进行更快地细胞分选。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。亦可选择即用型、符合伦理来源的人外周血中性粒细胞冻存产品。探索更多为您实验流程优化的其它产品，包括培养基、补充剂、抗体等。

在该EasySep™负选流程中，非目标细胞被抗体复合物与磁珠标记。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的中性粒细胞分离，接着简单地将目的细胞倾倒或吸取至一个新的试管中。磁珠分选最快仅需14分钟，目标中性粒细胞即可用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。本分离试剂盒兼容经HetaSep™（产品号 #07906）沉降法或红细胞裂解法处理的细胞样本。

该产品可替代EasySep™人中性粒细胞富集试剂盒 (产品号 #19257) 以进行更快地细胞分选。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。亦可选择即用型、符合伦理来源的人外周血中性粒细胞冻存产品。探索更多为您实验流程优化的其它产品，包括培养基、补充剂、抗体等。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • EasyPlate™ EasySep™ Magnet (Catalog #18102) • RoboSep™-S (Catalog #21000)

亚型
细胞分选试剂盒

细胞类型
粒细胞及其亚群

种属
人

样本来源
PMNC，Whole Blood

筛选方法
Negative

应用
细胞分选

品牌
EasySep，RoboSep

研究领域
免疫

查看更多显示更少

实验数据

Figure 1. Typical EasySep™ Human Neutrophil Isolation Profile

Starting with whole blood prepared using HetaSep™ or Lymphoprep™ with RBC lysis, the neutrophil content (CD45+CD16+CD66b+) of the isolated fraction typically ranges from 98.7 ± 0.9% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 52.0% and 99.0%, respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

RoboSep™ Human Neutrophil Isolation Kit

Catalog #

17957RF

Lot #

All

Language

English

Document Type

Product Information Sheet

Product Name

EasySep™ Human Neutrophil Isolation Kit

Catalog #

17957

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

RoboSep™ Human Neutrophil Isolation Kit

Catalog #

17957RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

RoboSep™ Human Neutrophil Isolation Kit

Catalog #

17957RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 3

Product Name

RoboSep™ Human Neutrophil Isolation Kit

Catalog #

17957RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

EasySep™ Human Neutrophil Isolation Kit

Catalog #

17957

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

EasySep™ Human Neutrophil Isolation Kit

Catalog #

17957

Lot #

All

Language

English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area

Workflow Stages

Workflow Stages for Granulocyte Research

Cell Sourcing

Cell Isolation

Cell Characterization

相关材料与文献

Dysfunction of low-density neutrophils in peripheral circulation in patients with sepsis. R. Sun et al. Scientific reports 2022 jan

Abstract

Low-density neutrophils (LDNs) have been described in tumors and various autoimmune diseases, where they exhibit immune dysfunction and alter disease progression. Nevertheless, LDNs have been rarely reported in sepsis. We studied sepsis patients admitted to the intensive care unit. Wright-Giemsa stain assay and Transmission electron microscopy were performed to detect the morphology of neutrophils. Flow cytometry was used to analyze the number and function of LDNs. Concentration of cytokines was measured using ELISA. Neutrophil chemotaxis was examined using an under-agarose chemotaxis model. We found that LDNs were significantly elevated in patients with sepsis. Phenotypes and morphological characteristics suggest that LDNs may be formed by mixtures of neutrophils at various maturation stages. In vitro experiments showed that LDN formation was closely associated with neutrophil degranulation. We preliminarily discussed changes in immune function in LDNs. Compared with high-density neutrophils, expression levels of CXC chemokine receptor 4 on LDN surfaces were increased, phagocytotic capacity was decreased, and life span was prolonged. The chemotactic ability of LDNs was significantly reduced, possibly related to the increased expression of P2X1. These data suggest that LDNs are essential components of neutrophils in sepsis. To clarify the source and dysfunction mechanism of LDN in sepsis may be helpful for the diagnosis and treatment of sepsis in the future.

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Exosomal transfer of activated neutrophil-derived lncRNA CRNDE promotes proliferation and migration of airway smooth muscle cells in asthma. X.-Y. Zhang et al. Human molecular genetics 2022 feb

Abstract

Activated neutrophil-derived exosomes reportedly contribute to the proliferation of airway smooth muscle cells (ASMCs), thereby aggravating the airway wall remodeling during asthma; however, the specific mechanism remains unclear. Lipopolysaccharide (LPS)-EXO and si-CRNDE-EXO were extracted from the media of human neutrophils treated with LPS and LPS??+??si-CRNDE (a siRNA targets long non-coding RNA CRNDE), respectively. Human ASMCs were co-cultured with LPS-EXO or si-CRNDE-EXO, and cell viability, proliferation and migration were measured. The interplay of colorectal neoplasia differentially expressed (CRNDE), inhibitor of nuclear factor kappa B kinase subunit beta (IKK$\beta$) and nuclear receptor subfamily 2 group C member 2 (TAK1) was explored using RNA immunoprecipitation (RIP) and Co-IP assays. A mouse model of asthma was induced using ovalbumin. CRNDE was upregulated in LPS-EXO and successfully transferred from LPS-treated neutrophils to ASMCs through exosome. Mechanically, CRNDE loaded in LPS-EXO reinforced TAK1-mediated IKK$\beta$ phosphorylation, thereby activating the nuclear factor kappa B (NF-$\kappa$B) pathway. Functionally, silencing CRNDE in LPS-EXO, an IKK$\beta$ inhibitor, and an NF-$\kappa$B inhibitor all removed the upregulation of cell viability, proliferation and migration induced by LPS-EXO in ASMCs. In the end, the in vivo experiment demonstrated that CRNDE knockdown in neutrophils effectively reduced the thickness of bronchial smooth muscle in a mouse model for asthma. Activated neutrophils-derived CRNDE was transferred to ASMCs through exosomes and activated the NF-$\kappa$B pathway by enhancing IKK$\beta$ phosphorylation. The latter promoted the proliferation and migration of ASMCs and then contributed to airway remodeling in asthma.

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Sputum from patients with primary ciliary dyskinesia contains high numbers of dysfunctional neutrophils and inhibits efferocytosis. M. Blanter et al. Respiratory research 2022 dec

Abstract

BACKGROUND Primary ciliary dyskinesia (PCD) is a genetic disorder characterized by recurrent airway infection and inflammation. There is no cure for PCD and to date there are no specific treatments available. Neutrophils are a crucial part of the immune system and are known to be dysfunctional in many inflammatory diseases. So far, the role of the neutrophils in PCD airways is largely unknown. The purpose of this study was to investigate the phenotype and function of airway neutrophils in PCD, and compare them to blood neutrophils. METHODS Paired peripheral blood and spontaneously expectorated sputum samples from patients with PCD (n??=??32) and a control group of patients with non-PCD, non-cystic fibrosis bronchiectasis (n??=??5) were collected. The expression of neutrophil-specific surface receptors was determined by flow cytometry. Neutrophil function was assessed by measuring the extent of actin polymerization, production of reactive oxygen species (ROS) and release of neutrophil extracellular traps (NETs) in response to activating stimuli. RESULTS Sputum neutrophils displayed a highly activated phenotype and were unresponsive to stimuli that would normally induce ROS production, actin polymerization and the expulsion of NETs. In addition, PCD sputum displayed high activity of neutrophil elastase, and impaired the efferocytosis by healthy donor macrophages. CONCLUSIONS Sputum neutrophils in PCD are dysfunctional and likely contribute to ongoing inflammation in PCD airways. Further research should focus on anti-inflammatory therapies and stimulation of efferocytosis as a strategy to treat PCD.

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EasySep™人中性粒细胞分选试剂盒

产品号 #（选择产品）

产品号 #17957_C

产品优势

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实验数据

产品说明书及文档

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Scientists Helping Scientists™

种属	Human
Magnet Compatibility	• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • EasyPlate™ EasySep™ Magnet (Catalog #18102) • RoboSep™-S (Catalog #21000)
样本来源	PMNC, Whole Blood
Selection Method	Negative

EasySep™人中性粒细胞分选试剂盒

产品号 #（选择产品）

产品号 #17957_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

技术资料 (10)

文献 (6)

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