Mouse Hepatic Progenitor Organoid Culture: Supplementary Protocols
This Technical Bulletin provides supplementary protocols for HepatiCult(tm) Organoid Growth Medium (Mouse), including organoid initiation and passaging from single cells and culturing hepatic progenitor organoids in a dilute matrix suspension. This document also includes general recommendations and photographs that illustrate key steps of hepatic progenitor organoid culture.
Passaging Naive PSC Colonies Derived and Maintained in RSeT™
How to passage naive colonies derived in RSeT™ without transferring irradiated mouse embryonic fibroblasts (iMEFs) to the next passage, and what you can expect during the early stages of reversion
Meritxell Huch, PhD
Dr. Meritxell Huch describes her work on tissue regeneration and developing tissue-specific disease models for the liver and pancreas
Caroline Lindemans, MD, PhD
Dr. Caroline Lindemans describes her research using organoids as a model system for intestinal regeneration and repair
Tamara Zietek, PhD
Dr. Tamara Zietek describes her work studying intestinal nutrient absorption with organoids
Joo-Hyeon Lee, PhD
Dr. Joo-Hyeon Lee shares her work using pulmonary organoids to study the cellular interactions between adult stem cells and niche cells in the lung
Jason Spence
Dr. Jason Spence describes his work on developing organoid systems to investigate tissue development and the physiology of immature tissues
Qi Tan, PhD
Dr. Qi Tan describes his work studying how epithelial cells maintain lung epithelial-mesenchymal homeostasis and protect the lung from fibrosis