Modeling Host-Microbe Interactions Using Human Intestinal Organoids
A common challenge to studying host-microbe interactions is the lack of optimal in vitro culture systems to recapitulate in vivo infection. In this virtual paper presentation, Dr. Devanjali Dutta discusses her research using human intestinal and lung organoids to study Cryptosporidium infection. Cryptosporidium is a protozoan parasite, a leading cause of diarrhea, and a major cause of child mortality worldwide. Dr. Dutta describes how she—and her colleagues in the lab of Dr. Hans Clevers—infected intestinal and lung organoids derived from healthy human donors with Cryptosporidium. In their paper*, organoids are presented as a physiologically relevant in vitro model to study Cryptosporidium and other pathogenic infections.
Dr. Devanjali Dutta is a Postdoctoral researcher in the lab of Dr. Hans Clever at the Hubrecht Institute.
*Heo I, Dutta D et al. (2018) Nat Microbiol. 3(7):814–23.
Organoid Expert Panel
What could organoids do for your research? In this in-depth panel discussion, STEMCELL's organoid experts answer questions submitted by you. Topics include evaluating organoids as tissue and disease models, variability in organoid cultures, and future outlooks in translational and clinical applications.
Heather McCauley, PhD
Dr. Heather McCauley describes her work investigating enteroendocrine regulation of nutrient absorption using PSC-derived human intestinal organoids
Dr. Jason Spence
Dr. Jason Spence shares his insights on the derivation of gastrointestinal organoids from hPSCs
Dr. Caroline Lindemans
Dr. Caroline Lindemans talks about her research on the role of innate immune cells in intestinal stem cell maintenance
Dr. Tamara Zietek
Dr. Tamara Zietek discusses the use of organoids to study nutrient sensing and transport by the intestinal epithelium
EasySep™小鼠TIL（CD45）正选试剂盒
How to Count Intestinal Crypts for Mouse Organoid Cultures How to count intestinal crypts when setting up organoid culture from mouse tissue
Modeling Host-Microbe Interactions Using Human Intestinal Organoids A common challenge to studying host-microbe interactions is the lack of optimal in vitro culture systems to recapitulate in vivo infection. In this virtual paper presentation, Dr. Devanjali Dutta discusses her research using human intestinal and lung organoids to study Cryptosporidium infection. Cryptosporidium is a protozoan parasite, a leading cause of diarrhea, and a major cause of child mortality worldwide. Dr. Dutta describes how she—and her colleagues in the lab of Dr. Hans Clevers—infected intestinal and lung organoids derived from healthy human donors with Cryptosporidium. In their paper*, organoids are presented as a physiologically relevant in vitro model to study Cryptosporidium and other pathogenic infections.
Supplementary Protocols for IntestiCult™ Organoid Growth Medium (Human) Protocols for the differentiation of human intestinal organoids, growth as a monolayer culture, and cryopreservation and thawing protocols for long-term storage.
Organoid Expert Panel What could organoids do for your research? In this in-depth panel discussion, STEMCELL's organoid experts answer questions submitted by you. Topics include evaluating organoids as tissue and disease models, variability in organoid cultures, and future outlooks in translational and clinical applications.
Heather McCauley, PhD Dr. Heather McCauley describes her work investigating enteroendocrine regulation of nutrient absorption using PSC-derived human intestinal organoids
Dr. Jason Spence Dr. Jason Spence shares his insights on the derivation of gastrointestinal organoids from hPSCs
Dr. Caroline Lindemans Dr. Caroline Lindemans talks about her research on the role of innate immune cells in intestinal stem cell maintenance
Dr. Tamara Zietek Dr. Tamara Zietek discusses the use of organoids to study nutrient sensing and transport by the intestinal epithelium
EMBL Organoids 2016 Conference Listen to interviews from the 2016 EMBL Organoids Meeting
Caroline Lindemans, MD, PhD Dr. Caroline Lindemans describes her research using organoids as a model system for intestinal regeneration and repair
