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Maintenance of Pluripotent Stem Cells
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Enhancing Genetic Stability in Human Pluripotent Stem Cells Maintained As Single Cells: Introducing eTeSR™ In this Innovation Showcase talk from ISSCR 2023, Dr. Adam Hirst, one of STEMCELL’s Senior Scientists, introduces eTeSR™, a novel hPSC maintenance medium developed specifically to maintain cell quality when passaging hPSCs as single cells. -
Scaling Biomimetic Culture of Pluripotent Stem Cells Hear from Dr. Maxime Feyeux, who discusses what the in vivo growth environment of pluripotent stem cells (PSCs) can teach us about in vitro culture. He summarizes some of the current challenges facing standard PSC culture technologies and shares a solution for mass producing high-quality PSCs for downstream applications. -
How to Count hPSC Aggregates to Determine Plating Density for Maintenance and Differentiation Step-by-step instructions for counting human pluripotent stem cell (hPSC) aggregates to determine and adjust the plating densities for hPSC maintenance and differentiation -
How to Passage hPSC Aggregates in mTeSR™3D Suspension Culture with a Clump Passaging Method Two-stage clump passaging protocol using the mTeSR™3D suspension culture system to minimize the risk of karyotype abnormalities that can be associated with single cell passaging -
Large-Scale Expansion of Human Pluripotent Stem Cells in 3D Suspension Culture with mTeSR™3D Medium Features and benefits of mTeSR™3D, a specialized medium for expansion of human pluripotent stem cells in suspension culture, including the novel fed-batch culture system
