Educational Materials
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Cleaning Your RoboSep™ Tip Head RoboSep™, fully automated cell separator, is virtually maintenance free, but it is vital to polish the tip head to ensure tips are stripped properly
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Neural Induction of hPSCs Using the Embryoid Body Method STEMdiff™ Neural Induction Medium is a defined, serum-free medium for the efficient & reproducible derivation of neural progenitor cells
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Generate Size-Controlled Spheroids and Embryoid Bodies Using AggreWell™ Microwell Plates AggreWell™ plates provide an easy and standardized approach to control size of embryoid bodies (EBs) for differentiation by adjusting initial cell input
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Coating Plates with Matrigel® for Pluripotent Stem Cell Culture Culturing and maintaining human ESCs and iPSCs with mTeSR™1 (Catalog #5850) or TeSR™2 (Catalog #05860) requires tissue culture plates coated with a matrix
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Vitronectin XF™ Defined Matrix for Human Pluripotent Stem Cells Technical tip from our dedicated team of Product and Scientific Support specialists
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Tap Your Way to Easy Pluripotent Stem Cell Passaging Using ReLeSR™ Technical tip from our dedicated team of Product and Scientific Support specialists
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Tips for Reprogramming Somatic Cells using TeSR™-E7™ Tips to help resolve 2 common issues encountered when reprogramming fibroblasts using TeSR™-E7™
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Optimization of ALDEFLUOR™ Assay for Detection of ALDH Activity in Your Cell Type of Interest ALDEFLUOR™ reagent system is a non-immunological method to identify human stem and progenitor cells based on their aldehyde dehydrogenase (ALDH) activity
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DNase I Treatment for Clumpy Cell Suspensions To reduce cell clumping and minimize presence of free-floating DNA fragments, we recommend adding DNase I Solution (Catalog #07900) to the cell suspension
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Creating a Hypoxic Environment for Cell Culture For inducing low oxygen tension for cell culture, STEMCELL offers the Hypoxia Incubator Chamber (Catalog #27310)
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How to Reduce Hematopoietic Cell Contamination in Primary Mouse Mesenchymal Stem Cell Cultures MesenCult™ Proliferation Kit with MesenPure™ reduces hematopoietic cell contamination in primary mouse mesenchymal stem cell cultures
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Don't Want to Use Serum? Alternatives for Cell Separation Buffers When isolating cells using EasySep™ or RoboSep™ cell separation systems, the recommended buffer is 2% FBS in Phosphate Buffered Saline w/ 1 mM EDTA