Tools for Optimizing Human Immune Cell Research
Obtaining consistent and reliable results when culturing immune cells can be challenging. Watch this webinar to discover how to obtain high yields of functional T cells, NK cells, B cells, dendritic cells, and macrophages for your research applications. The speaker, Evan Karas, also explains how to expand primary T cells without feeders or serum.
Harvesting cells from CFU assays
Cells from colonies grown in methylcellulose-based medium can be harvested for further analysis or culture by plucking or washing them out of the medium
Implementing the Colony-Forming Unit (CFU) Assay As a Potency Assay for Hematopoietic Cell Therapy Products
Assessing the functional potency of cells for use in cell therapy research presents unique challenges. For hematopoietic stem and progenitor cells (HSPCs) in particular, potency can be measured in vitro by assessing the ability of these cells to differentiate into progenitor cells using the colony-forming unit (CFU) assay. Validating the CFU assay as a potency assay requires demonstrating its specificity, accuracy, precision, linearity, and reproducibility. Once validated, the CFU assay can assess the quality and consistency of prospective hematopoietic cell therapy products (HCTPs) at multiple stages of the processing and manufacturing workflow.
Join Dr. Colin Hammond and learn about the regulatory guidance around the potency testing of HCTPs and how to validate the CFU assay as a potency assay that can be integrated into cell therapy manufacturing workflows.
EasySep™小鼠TIL（CD45）正选试剂盒
How to Prepare and Plate Semi-Solid Medium Steps involved with preparing and plating methylcellulose-based semi-solid media (e.g. MethoCult™ and ClonaCell™)
Serum-Free Expansion of CD34+ Cells with SFEM II and UM729 Explore the use of StemSpan™ SFEM II medium, StemSpan™ CD34+ Expansion Supplement and small molecules UM171 or UM729 to enhance expansion of HSPCs
Tools for Optimizing Human Immune Cell Research Obtaining consistent and reliable results when culturing immune cells can be challenging. Watch this webinar to discover how to obtain high yields of functional T cells, NK cells, B cells, dendritic cells, and macrophages for your research applications. The speaker, Evan Karas, also explains how to expand primary T cells without feeders or serum.
Human Hematopoietic Stem and Progenitor Cell Markers and Phenotyping Panels Technical tip from our dedicated team of Product and Scientific Support specialists
Harvesting cells from CFU assays Cells from colonies grown in methylcellulose-based medium can be harvested for further analysis or culture by plucking or washing them out of the medium
Implementing the Colony-Forming Unit (CFU) Assay As a Potency Assay for Hematopoietic Cell Therapy Products Assessing the functional potency of cells for use in cell therapy research presents unique challenges. For hematopoietic stem and progenitor cells (HSPCs) in particular, potency can be measured in vitro by assessing the ability of these cells to differentiate into progenitor cells using the colony-forming unit (CFU) assay. Validating the CFU assay as a potency assay requires demonstrating its specificity, accuracy, precision, linearity, and reproducibility. Once validated, the CFU assay can assess the quality and consistency of prospective hematopoietic cell therapy products (HCTPs) at multiple stages of the processing and manufacturing workflow.
Tips for Reprogramming Somatic Cells using TeSR™-E7™ Tips to help resolve 2 common issues encountered when reprogramming fibroblasts using TeSR™-E7™
