Studying Cystic Fibrosis Using Primary Human Nasal Epithelial Cells
Cystic Fibrosis (CF) is a genetic disease that affects multiple organs in the body. The primary defects are in the airway and related to epithelial dysfunction. Technological advances have enabled researchers to study CF using specialized in vitro cell culture models to recapitulate the pseudostratified airway epithelium. In this webinar, Dr. Theo Moraes elaborates on the advantages of using nasal epithelial cell cultures to perform such studies and their implications for precision medicine in CF. He also discusses the Program for Individualized Cystic Fibrosis Therapy (CFIT)—a collaboration between CF Canada, SickKids Foundation, and The Hospital for Sick Children—and how it utilizes the nasal culture methodology to develop a nationally accessible resource and to accelerate the development of individualized therapies for CF patients.
Dr. Theo Moraes is a clinician and researcher at The Hospital for Sick Children and the SickKids Research Institute in Toronto, Canada.
Modeling Host-Microbe Interactions Using Human Intestinal Organoids
A common challenge to studying host-microbe interactions is the lack of optimal in vitro culture systems to recapitulate in vivo infection. In this virtual paper presentation, Dr. Devanjali Dutta discusses her research using human intestinal and lung organoids to study Cryptosporidium infection. Cryptosporidium is a protozoan parasite, a leading cause of diarrhea, and a major cause of child mortality worldwide. Dr. Dutta describes how she—and her colleagues in the lab of Dr. Hans Clevers—infected intestinal and lung organoids derived from healthy human donors with Cryptosporidium. In their paper*, organoids are presented as a physiologically relevant in vitro model to study Cryptosporidium and other pathogenic infections.
Dr. Devanjali Dutta is a Postdoctoral researcher in the lab of Dr. Hans Clever at the Hubrecht Institute.
*Heo I, Dutta D et al. (2018) Nat Microbiol. 3(7):814–23.
Organoid Expert Panel
What could organoids do for your research? In this in-depth panel discussion, STEMCELL's organoid experts answer questions submitted by you. Topics include evaluating organoids as tissue and disease models, variability in organoid cultures, and future outlooks in translational and clinical applications.
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Studying Cystic Fibrosis Using Primary Human Nasal Epithelial Cells Cystic Fibrosis (CF) is a genetic disease that affects multiple organs in the body. The primary defects are in the airway and related to epithelial dysfunction. Technological advances have enabled researchers to study CF using specialized in vitro cell culture models to recapitulate the pseudostratified airway epithelium. In this webinar, Dr. Theo Moraes elaborates on the advantages of using nasal epithelial cell cultures to perform such studies and their implications for precision medicine in CF. He also discusses the Program for Individualized Cystic Fibrosis Therapy (CFIT)—a collaboration between CF Canada, SickKids Foundation, and The Hospital for Sick Children—and how it utilizes the nasal culture methodology to develop a nationally accessible resource and to accelerate the development of individualized therapies for CF patients.
Modeling Host-Microbe Interactions Using Human Intestinal Organoids A common challenge to studying host-microbe interactions is the lack of optimal in vitro culture systems to recapitulate in vivo infection. In this virtual paper presentation, Dr. Devanjali Dutta discusses her research using human intestinal and lung organoids to study Cryptosporidium infection. Cryptosporidium is a protozoan parasite, a leading cause of diarrhea, and a major cause of child mortality worldwide. Dr. Dutta describes how she—and her colleagues in the lab of Dr. Hans Clevers—infected intestinal and lung organoids derived from healthy human donors with Cryptosporidium. In their paper*, organoids are presented as a physiologically relevant in vitro model to study Cryptosporidium and other pathogenic infections.
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Organoid Expert Panel What could organoids do for your research? In this in-depth panel discussion, STEMCELL's organoid experts answer questions submitted by you. Topics include evaluating organoids as tissue and disease models, variability in organoid cultures, and future outlooks in translational and clinical applications.
