Tech Tips

Removing Granulocytes from Old Blood Samples Technical tip from our dedicated team of Product and Scientific Support specialists

Considerations for Flow Cytometry Gating To achieve accurate results, it is important to use an optimal gating strategy when assessing cell samples via flow cytometry

Preparing a Polymorphonuclear Cell Fraction from Whole Blood using Ammonium Chloride Lysis Technical tip from our dedicated team of Product and Scientific Support specialists

Reducing Platelet Contamination in Blood Samples Platelets (thrombocytes), cell fragments derived from megakaryocytes, are used in normal blood clotting, and must be handled gently to prevent activation

Critical Points for Culturing Human Bronchial Epithelial Cells at Air-Liquid Interface Primary human bronchial epithelial cells (HBECs) can be differentiated to a physiologically relevant mucociliated phenotype using PneumaCult™-ALI

Total Nucleated Cell and Viable Cell Counts Accurate cell counts are important in experiments. Ideally both the total nucleated cell (TNC) count and the viability of the cells should be assessed

Using Cell Density Gradients to Optimize Your Cell Isolations RosetteSep™, a unique immunodensity cell isolation platform, isolates highly purified cells by density gradient centrifugation without columns or magnets

Maintaining Humidity in CFU Assays using MethoCult™ Media Technical tip from our dedicated team of Product and Scientific Support specialists

Calculating Cell Concentration for Plating CFU Assays Following RBC Clearance with HetaSep™ When preparing small volume samples of fresh blood for CFU assays, we recommend HetaSep™ (Catalog #7806/7906) to remove red blood cells prior to plating

Harvesting cells from CFU assays Cells from colonies grown in methylcellulose-based medium can be harvested for further analysis or culture by plucking or washing them out of the medium

Colony-Forming Unit Assays with Mouse Cells STEMCELL Technologies offers a variety of MethoCult methylcellulose-based semi-solid media products to be used with mouse cells for colony forming unit (CFU) assays

Expansion of CD34+ Cells in Serum-Free Culture Technical tip from our dedicated team of Product and Scientific Support specialists