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EasySep™人初始CD4+ T细胞分选试剂盒II

免疫磁珠阴性分选法获取未被标记的人初始CD4+ T细胞
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产品号 #17555_C

免疫磁珠阴性分选法获取未被标记的人初始CD4+ T细胞

产品优势

  • 操作简单、快捷，且无需分离柱
  • 纯度可高达98%
  • 获得的活细胞未被标记

产品组分包括

  • EasySep™人Naïve CD4+ T细胞分选试剂盒 II（产品号 #17555）
    • EasySep™人Naïve CD4+ T细胞分选抗体混合物II，1mL
    • EasySep™ Dextran RapidSpheres™磁珠，1mL
    • RoboSep™ 人Naïve CD4+ T细胞分选试剂盒II    
    • EasySep™人Naïve CD4+ T细胞分选抗体混合物II,1mL
    • EasySep™ Dextran RapidSpheres™磁珠，1mL
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（目录编号#20125）
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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用EasySep™人初始CD4+ T细胞分离试剂盒II，通过免疫磁珠负选法，可轻松高效地从新鲜或冻存的人外周血单个核细胞(PBMC)样本中分离高纯度初始CD4+ T细胞(CD3+CD4+CD45RA+CD45RO-)。EasySep™技术结合单克隆抗体的特异性和免分离柱系统的简便性，已在发表的研究中广泛应用超过20年。

本EasySep™阴性分选方案中，非目标细胞通过抗体复合物与磁珠标记。表达以下标记物的非目标细胞将被去除：CD2、CD14、CD16、CD19、CD20、CD25、CD36、CD45RO、CD56、CD61、CD66b、CD123、GlyA、TCRγδ和HLA-DR。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着仅需简单 将目的细胞倾倒或枪头吸取至新的管中。仅需短至11分钟的磁珠分选，即可获得适用于流式细胞术、细胞培养或DNA/RNA提取等下游应用的初始CD4+ T细胞。

本试剂盒取代EasySep™人初始CD4+ T细胞富集试剂盒(目录号19155)和EasySep™人初始CD4+ T细胞分离试剂盒(目录号19555)，可实现更快速的细胞分选     。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。或选择即用型、符合伦理来源的EasySep™人初始CD4+ T细胞分离试剂盒II分离的冻存人外周血CD4+CD45RA+ 初始CD4+T细胞。探索更多产品，优化您的实验流程，包括培养基、添加剂、抗体等。

本EasySep™阴性分选方案中，非目标细胞通过抗体复合物与磁珠标记。表达以下标记物的非目标细胞将被去除：CD2、CD14、CD16、CD19、CD20、CD25、CD36、CD45RO、CD56、CD61、CD66b、CD123、GlyA、TCRγδ和HLA-DR。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着仅需简单 将目的细胞倾倒或枪头吸取至新的管中。仅需短至11分钟的磁珠分选，即可获得适用于流式细胞术、细胞培养或DNA/RNA提取等下游应用的初始CD4+ T细胞。

本试剂盒取代EasySep™人初始CD4+ T细胞富集试剂盒(目录号19155)和EasySep™人初始CD4+ T细胞分离试剂盒(目录号19555)，可实现更快速的细胞分选     。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。或选择即用型、符合伦理来源的EasySep™人初始CD4+ T细胞分离试剂盒II分离的冻存人外周血CD4+CD45RA+ 初始CD4+T细胞。探索更多产品，优化您的实验流程，包括培养基、添加剂、抗体等。

本EasySep™阴性分选方案中，非目标细胞通过抗体复合物与磁珠标记。表达以下标记物的非目标细胞将被去除：CD2、CD14、CD16、CD19、CD20、CD25、CD36、CD45RO、CD56、CD61、CD66b、CD123、GlyA、TCRγδ和HLA-DR。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着仅需简单 将目的细胞倾倒或枪头吸取至新的管中。仅需短至11分钟的磁珠分选，即可获得适用于流式细胞术、细胞培养或DNA/RNA提取等下游应用的初始CD4+ T细胞。

本试剂盒取代EasySep™人初始CD4+ T细胞富集试剂盒(目录号19155)和EasySep™人初始CD4+ T细胞分离试剂盒(目录号19555)，可实现更快速的细胞分选     。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。或选择即用型、符合伦理来源的EasySep™人初始CD4+ T细胞分离试剂盒II分离的冻存人外周血CD4+CD45RA+ 初始CD4+T细胞。探索更多产品，优化您的实验流程，包括培养基、添加剂、抗体等。

本EasySep™阴性分选方案中，非目标细胞通过抗体复合物与磁珠标记。表达以下标记物的非目标细胞将被去除：CD2、CD14、CD16、CD19、CD20、CD25、CD36、CD45RO、CD56、CD61、CD66b、CD123、GlyA、TCRγδ和HLA-DR。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着仅需简单 将目的细胞倾倒或枪头吸取至新的管中。仅需短至11分钟的磁珠分选，即可获得适用于流式细胞术、细胞培养或DNA/RNA提取等下游应用的初始CD4+ T细胞。

本试剂盒取代EasySep™人初始CD4+ T细胞富集试剂盒(目录号19155)和EasySep™人初始CD4+ T细胞分离试剂盒(目录号19555)，可实现更快速的细胞分选     。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。或选择即用型、符合伦理来源的EasySep™人初始CD4+ T细胞分离试剂盒II分离的冻存人外周血CD4+CD45RA+ 初始CD4+T细胞。探索更多产品，优化您的实验流程，包括培养基、添加剂、抗体等。

本EasySep™阴性分选方案中，非目标细胞通过抗体复合物与磁珠标记。表达以下标记物的非目标细胞将被去除：CD2、CD14、CD16、CD19、CD20、CD25、CD36、CD45RO、CD56、CD61、CD66b、CD123、GlyA、TCRγδ和HLA-DR。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着仅需简单将目的细胞倾倒或枪头吸取至新的管中。仅需短至11分钟的磁珠分选，即可获得适用于流式细胞术、细胞培养或DNA/RNA提取等下游应用的初始CD4+ T细胞。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
T 细胞，T 细胞，CD4+
 
种属
人
 
样本来源
PBMC
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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实验数据

Figure 1. Typical EasySep™ Human Naive CD4 T Cell Isolation Profile

Starting with fresh mononuclear cells, the naïve CD4+ T cell content (CD3+CD4+CD45RA+CD45RO-) of the isolated fraction is typically 96.6 ± 1.5% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 13.0% and 97.3%, respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Naïve CD4+ T Cell Isolation Kit II
Catalog #
17555RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Human Naïve CD4+ T Cell Isolation Kit II
Catalog #
17555
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Naïve CD4+ T Cell Isolation Kit II
Catalog #
17555RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Naïve CD4+ T Cell Isolation Kit II
Catalog #
17555RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Naïve CD4+ T Cell Isolation Kit II
Catalog #
17555RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Naïve CD4+ T Cell Isolation Kit II
Catalog #
17555
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Naïve CD4+ T Cell Isolation Kit II
Catalog #
17555
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (9)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Antigen Processing and Presentation
Wallchart
Antigen Processing and Presentation
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...

文献 (7)

Soluble RAGE Prevents Type 1 Diabetes Expanding Functional Regulatory T Cells. S. S. Leung et al. Diabetes 2022 sep

Abstract

Type 1 diabetes is an autoimmune disease with no cure, where clinical translation of promising therapeutics has been hampered by the reproducibility crisis. Here, short-term administration of an antagonist to the receptor for advanced glycation end products (sRAGE) protected against murine diabetes at two independent research centers. Treatment with sRAGE increased regulatory T cells (Tregs) within the islets, pancreatic lymph nodes, and spleen, increasing islet insulin expression and function. Diabetes protection was abrogated by Treg depletion and shown to be dependent on antagonizing RAGE with use of knockout mice. Human Tregs treated with a RAGE ligand downregulated genes for suppression, migration, and Treg homeostasis (FOXP3, IL7R, TIGIT, JAK1, STAT3, STAT5b, CCR4). Loss of suppressive function was reversed by sRAGE, where Tregs increased proliferation and suppressed conventional T-cell division, confirming that sRAGE expands functional human Tregs. These results highlight sRAGE as an attractive treatment to prevent diabetes, showing efficacy and reproducibility at multiple research centers and in human T cells.
View publication
Blocking CCL8-CCR8-Mediated Early Allograft Inflammation Improves Kidney Transplant Function. A. Dangi et al. Journal of the American Society of Nephrology : JASN 2022 oct

Abstract

BACKGROUND In kidney transplantation, early allograft inflammation impairs long-term allograft function. However, precise mediators of early kidney allograft inflammation are unclear, making it challenging to design therapeutic interventions. METHODS We used an allogeneic murine kidney transplant model in which CD45.2 BALB/c kidneys were transplanted to CD45.1 C57BL/6 recipients. RESULTS Donor kidney resident macrophages within the allograft expanded rapidly in the first 3 days. During this period, they were also induced to express a high level of Ccl8, which, in turn, promoted recipient monocyte graft infiltration, their differentiation to resident macrophages, and subsequent expression of Ccl8. Enhanced graft infiltration of recipient CCR8+ T cells followed, including CD4, CD8, and ?? T cells. Consequently, blocking CCL8-CCR8 or depleting donor kidney resident macrophages significantly inhibits early allograft immune cell infiltration and promotes superior short-term allograft function. CONCLUSIONS Targeting the CCL8-CCR8 axis is a promising measure to reduce early kidney allograft inflammation.
View publication
Age-associated impairment of T cell immunity is linked to sex-dimorphic elevation of N-glycan branching. H. Mkhikian et al. Nature aging 2022 mar

Abstract

Impaired T cell immunity with aging increases mortality from infectious disease. The branching of Asparagine-linked glycans is a critical negative regulator of T cell immunity. Here we show that branching increases with age in females more than males, in na{\{i}}ve more than memory T cells and in CD4+ more than CD8+ T cells. Female sex hormones and thymic output of na{\"{i}}ve T cells (TN) decrease with age however neither thymectomy nor ovariectomy altered branching. Interleukin-7 (IL-7) signaling was increased in old female more than male mouse TN cells and triggered increased branching. N-acetylglucosamine a rate-limiting metabolite for branching increased with age in humans and synergized with IL-7 to raise branching. Reversing elevated branching rejuvenated T cell function and reduced severity of Salmonella infection in old female mice. These data suggest sex-dimorphic antagonistic pleiotropy where IL-7 initially benefits immunity through TN maintenance but inhibits TN function by raising branching synergistically with age-dependent increases in N-acetylglucosamine."
View publication
View All Publications

更多信息

更多信息
种属 Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源 PBMC
Selection Method Negative
标记抗体
  1. 抗人CD4抗体，克隆OKT4
    抗人CD4抗体，克隆OKT4

    小鼠单克隆IgG2b抗体，抗人、恒河猴、食蟹猴CD4

  2. 抗人CD45抗体，克隆HI30
    抗人CD45抗体，克隆HI30

    小鼠单克隆IgG1抗体，抗人、黑猩猩CD45

  3. 抗人CD45RA，克隆HI100
    抗人CD45RA，克隆HI100

    抗人、黑猩猩CD45RA的小鼠单克隆IgG2b抗体

  4. 抗人CD45RO抗体，克隆UCHL1
    抗人CD45RO抗体，克隆UCHL1

    小鼠单克隆IgG2a抗体，抗人、黑猩猩、普通狨猴CD45RO

  5. 抗人CD3抗体，克隆UCHT1
    抗人CD3抗体，克隆UCHT1

    小鼠（BALB/c）单克隆IgG1抗体，抗人、黑猩猩CD3

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