产品号 #19863_C
免疫磁珠负选试剂盒
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免疫磁珠负选试剂盒
EasySep™ 试剂盒含RoboSep™缓冲液和RoboSep™过滤吸头
免疫磁珠负选试剂盒
EasySep™ 小鼠Pan-DC富集试剂盒 II 旨在通过免疫磁珠负选,从小鼠脾细胞中分离树突细胞(DCs)。在该EasySep™分选流程中,非目的细胞被抗体复合物与磁珠标记。通过EasySep™磁极将标记的细胞与未被标记的目的细胞分离,接着简单地将目的细胞倾倒或吸取至一个新的试管中。
本产品替代了 EasySep™ 小鼠Pan-DC富集试剂盒(产品号 #19763),可提高得率并实现更快速的细胞分选。
磁极兼容性
• EasySep™磁极(产品号 #18000)
• “The Big Easy” EasySep™磁极(产品号 #18001)
• EasyEights™ EasySep™磁极(产品号 #18103)
• RoboSep™-S(产品号 #21000)
分类
细胞分选试剂盒
细胞类型
树突状细胞(DCs)
种属
小鼠
样本来源
脾脏
分选方法
负选
应用
细胞分选
品牌
EasySep,RoboSep
研究领域
免疫,感染性疾病(传染病)
Figure 1. Typical EasySep™ Mouse Pan-DC Enrichment Profile
Starting with mouse splenocytes, the dendritic cell content (CD11c+lineage-) of the isolated fraction is typically 57.3 ± 5.5% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final enriched fractions are 3.9% and 62.4%, respectively.
Figure 2. Pre-enrichment with EasySep™ Reduces Sorting Time
Starting with mouse splenocytes, DCs and DC subsets were analyzed in parallel from unenriched or EasySep™-enriched samples by flow cytometry. Each sample was run for an equivalent amount of time with a consistent flow rate and the data was used to extrapolate the time required to sort 1 million pan-DCs, conventional DCs (cDCs), or plamacytoid DCs (pDCs). In all cases, the required FACS times were reduced with EasySep™-enriched samples compared to unenriched samples (n = 2, SD).
请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。
本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。
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种属 | Mouse |
---|---|
Magnet Compatibility | • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000) |
样本来源 | Spleen |
Selection Method | Negative |
小鼠脾脏消化解离培养基
免疫磁珠正选小鼠CD11c+细胞
免疫磁珠负选试剂盒
大鼠单克隆IgG2a抗体,抗小鼠CD19
亚美尼亚仓鼠单克隆IgG1抗体,抗小鼠CD3e
大鼠单克隆IgG2a抗体,抗小鼠F4/80
大鼠单克隆IgG2a抗体,抗小鼠Ly-6G
抗小鼠 NK1.1 (CD161) 的 (C3H x BALB/c) F1 杂交单克隆IgG2a抗体
抗小鼠TER119的大鼠(WI)单克隆IgG2b抗体
抗小鼠CD11c的仓鼠(亚美尼亚)单克隆IgG抗体
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