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EasySep™小鼠Pan-DC富集试剂盒II

免疫磁珠负选试剂盒

产品号 #(选择产品)

产品号 #19863_C

免疫磁珠负选试剂盒

产品优势

  • 操作简单、快捷,且无需分离柱
  • 纯度高达65%
  • 分选得到的细胞不带标记
  • 为高得率进行优化
  • 便于快速流式分选DC亚型

产品组分包括

  • EasySep™小鼠Pan-DC富集试剂盒(产品号 #19863)
    • EasySep™小鼠Pan-DC富集抗体混合物II,1 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001磁珠,1 mL
专为您的实验方案打造的产品

总览

EasySep™ 小鼠Pan-DC富集试剂盒 II 旨在通过免疫磁珠负选,从小鼠脾细胞中分离树突细胞(DCs)。在该EasySep™分选流程中,非目的细胞被抗体复合物与磁珠标记。通过EasySep™磁极将标记的细胞与未被标记的目的细胞分离,接着简单地将目的细胞倾倒或吸取至一个新的试管中。

本产品替代了 EasySep™ 小鼠Pan-DC富集试剂盒(产品号 #19763),可提高得率并实现更快速的细胞分选。

本产品替代了 EasySep™ 小鼠Pan-DC富集试剂盒(产品号 #19763),可提高得率并实现更快速的细胞分选。

本产品替代了 EasySep™ 小鼠Pan-DC富集试剂盒(产品号 #19763),可提高得率并实现更快速的细胞分选。

本产品替代了 EasySep™ 小鼠Pan-DC富集试剂盒(产品号 #19763),可提高得率并实现更快速的细胞分选。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
树突状细胞(DCs)
 
种属
小鼠
 
样本来源
Spleen
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
EasySep,RoboSep
 
研究领域
免疫,感染性疾病(传染病)
 

实验数据

Flow Cytometry Profile Results with EasySep™ Mouse Pan-DC Enrichment Kit II

Figure 1. Typical EasySep™ Mouse Pan-DC Enrichment Profile

Starting with mouse splenocytes, the dendritic cell content (CD11c+lineage-) of the isolated fraction is typically 57.3 ± 5.5% (mean ± SD using the purple EasySep™ magnet). In the above example, the purities of the start and final enriched fractions are 3.9% and 62.4%, respectively.

Reduce sort time using EasySep™ Mouse Pan-DC Enrichment Kit II

Figure 2. Pre-enrichment with EasySep™ Reduces Sorting Time

Starting with mouse splenocytes, DCs and DC subsets were analyzed in parallel from unenriched or EasySep™-enriched samples by flow cytometry. Each sample was run for an equivalent amount of time with a consistent flow rate and the data was used to extrapolate the time required to sort 1 million pan-DCs, conventional DCs (cDCs), or plamacytoid DCs (pDCs). In all cases, the required FACS times were reduced with EasySep™-enriched samples compared to unenriched samples (n = 2, SD).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
19863
Lot #
All
Language
English
Catalog #
19863RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
19863
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
19863
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
19863RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
19863RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
19863RF
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

更多信息

更多信息
种属 Mouse
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源 Spleen
Selection Method Negative
标记抗体
质量保证:

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