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ISSCR Standards in Action: Advancements in Cell Line Generation and Organoid Innovation

Predictive In Vitro Models for Toxicity Testing and Liver Research Having validated tools to perform predictive assays in a biologically-relevant system is key to success in drug discovery research. In this webinar, Dr. Terry Riss (Promega Corp) and Jennifer Christie (STEMCELL Technologies) discuss products and protocols that enable the incorporation of validated in vitro alternatives to animal models for toxicology studies. First, Dr. Riss presents in vitro assays for 3D cultures that monitor drug-induced cytotoxicity and explains how different assays can be multiplexed to measure multiple cell health parameters. Following this, Ms. Christie describes how to generate mature and functional hepatic organoids using HepatiCult™ and demonstrates their applications in liver research and hepatotoxicity assessment.

Passaging Intestinal Organoids into Suspension Culture Using IntestiCult™

Cryogenic Tissue Processing and Section Immunofluorescence of Cerebral Organoids

How to Culture Mouse Intestinal Organoids: Introduction Learn how to isolate mouse intestinal crypts and culture intestinal organoids using IntestiCult™ Organoid Growth Medium (Mouse) and establish organoid cultures in less than a week using this complete and defined medium

Optimization of EasySep™ Releasable RapidSpheres™ Indirect Positive Selection Kits Technical tip from our dedicated team of Product and Scientific Support specialists

Customizable Semi-Solid Cloning of Mammalian Cells in 96-Well Plates Using ClonaCell™ FLEX Medium ClonaCell™ FLEX 96-well plate semi-solid cloning protocol, including plating, liquid medium overlay, screening, and colony picking

When to use NeuroFluor™ CDr3 versus NeuroFluor™ NeuO? Technical tip from our dedicated team of Product and Scientific Support specialists

How to Generate Uniform, Size-Controlled 3D Spheroid Cultures with AggreWell™ Microwell Plates Learn how AggreWell™ Microwell Plates work to generate large numbers of uniform, size-controlled 3D cell culture spheroids for many areas of research, including cancer, stem cells, drug discovery and more

ClonaCell™-HY Procedure Selection and growth of mouse hybridomas to generate mouse monoclonal antibodies using ClonaCell™-HY including preparation of myeloma cells and splenocytes, fusion, selection and cloning, and harvesting

Forskolin-Induced Swelling of Airway Organoids Derived from Healthy and Cystic Fibrosis Donors

How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol Easy and rapid procedure for isolating peripheral blood mononuclear cells (PBMCs) from whole blood or bone marrow using the unique SepMate™ PBMC isolation tubes