Scientific Resources

Critical Points for Culturing Human Bronchial Epithelial Cells at Air-Liquid Interface Primary human bronchial epithelial cells (HBECs) can be differentiated to a physiologically relevant mucociliated phenotype using PneumaCult™-ALI
Total Nucleated Cell and Viable Cell Counts Accurate cell counts are important in experiments. Ideally both the total nucleated cell (TNC) count and the viability of the cells should be assessed
Using Cell Density Gradients to Optimize Your Cell Isolations RosetteSep™, a unique immunodensity cell isolation platform, isolates highly purified cells by density gradient centrifugation without columns or magnets
Maintaining Humidity in CFU Assays using MethoCult™ Media Technical tip from our dedicated team of Product and Scientific Support specialists
Harvesting cells from CFU assays Cells from colonies grown in methylcellulose-based medium can be harvested for further analysis or culture by plucking or washing them out of the medium
Colony-Forming Unit Assays with Mouse Cells STEMCELL Technologies offers a variety of MethoCult methylcellulose-based semi-solid media products to be used with mouse cells for colony forming unit (CFU) assays
Expansion of CD34+ Cells in Serum-Free Culture Technical tip from our dedicated team of Product and Scientific Support specialists
How to Isolate Mesenchymal Stromal Cells (MSCs) from Mouse Compact Bone This instructional video provides a step-by-step procedure on how to isolate mesenchymal stromal cells (MSCs) from mouse compact bone to achieve high yield and viability of MSCs
Semi-Solid Cloning of CHO Cells Using ClonaCell™-CHO Medium Procedure for semi-solid cloning, including preparation of the cells, plating, colony picking, and expansion
Mammalian Cell Cloning Procedure: Customizable Semi-Solid Cloning with ClonaCell™ FLEX Medium This video outlines the ClonaCell™ FLEX procedure for semi-solid cloning, including preparation of the cells, plating, colony picking, and expansion
Caring for your RoboSep™ Basic overview on how to use and maintain your RoboSep™
How to Pipette Off Samples During Cell Isolation Using the EasyEights™ EasySep™ Cell Separation Magnet Demonstrates the proper technique for pipetting off samples, to achieve optimal results when using the EasyEights™ EasySep™ magnet to isolate cells