Immunology Research

DNase I Treatment for Clumpy Cell Suspensions To reduce cell clumping and minimize presence of free-floating DNA fragments, we recommend adding DNase I Solution (Catalog #07900) to the cell suspension
Isolating Cells from Atypical Samples Technical tip from our dedicated team of Product and Scientific Support specialists
Assessment of Your Starting Sample and Calculation of Recovery from Cell Isolation Procedures Setting aside a small portion of your starting sample allows you to assess the frequency of desired cells in the sample prior to isolation
Isolate Multiple Cell Types from a Single Sample with Sequential Isolation Sequential separation, for higher recovery of purified cells from limited sample size and isolation of multiple cell types, is ideal for chimerism analysis
Importance of Using an FcR Blocker During Cell Separation Procedures EasySep™ Other Species indirect selection kits can be used to isolate cells from any species
Acquiring DNA or RNA from Positively Selected Cells Cells positvely selected with the EasySep™ system do not interfere with most downstream applications
Removing Granulocytes from Old Blood Samples Technical tip from our dedicated team of Product and Scientific Support specialists
Considerations for Flow Cytometry Gating To achieve accurate results, it is important to use an optimal gating strategy when assessing cell samples via flow cytometry
Preparing a Polymorphonuclear Cell Fraction from Whole Blood using Ammonium Chloride Lysis Technical tip from our dedicated team of Product and Scientific Support specialists
Reducing Platelet Contamination in Blood Samples Platelets (thrombocytes), cell fragments derived from megakaryocytes, are used in normal blood clotting, and must be handled gently to prevent activation
Total Nucleated Cell and Viable Cell Counts Accurate cell counts are important in experiments. Ideally both the total nucleated cell (TNC) count and the viability of the cells should be assessed
Using Cell Density Gradients to Optimize Your Cell Isolations RosetteSep™, a unique immunodensity cell isolation platform, isolates highly purified cells by density gradient centrifugation without columns or magnets