EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #19842_C
免疫磁珠负选细胞分选试剂盒
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总览
EasySep™小鼠ILC2富集试剂盒通过负选法，从肺组织或其他组织的单细胞悬液中富集ILC2（2型先天性淋巴细胞）。利用针对非ILC细胞的生物素化抗体和链霉亲和素包被的磁珠（RapidSpheres™），来去除不需要的细胞。通过EasySep™磁极分离被标记的细胞，无需使用分离柱。
磁极兼容性
• EasySep™磁极（产品号 #18000）
分类
细胞分选试剂盒
细胞类型
先天性淋巴细胞
种属
小鼠
样本来源
其它细胞系
分选方法
负选
应用
细胞分选
品牌
EasySep
研究领域
免疫
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
文献 (2)
Isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of PDAC. STAR protocols 2022 sep
Abstract
Innate lymphoid cells 2 (ILC2) play a significant role in the tumorigenesis of pancreatic ductal adenocarcinoma (PDAC). An important aspect of ILC2-mediated tumorigenesis is the expansion of the resident ILC2 and simultaneous recruitment of the peripheral ILC2. Here, we describe a protocol for isolation, enrichment, and DiD labeling of ILC2 for in vivo tracking of ILC2s in the mouse. Further, we describe steps for the adoptive transfer of ILC2 to a recipient mouse model of PDAC. For complete details on the use and execution of this protocol, please refer to Alam et al. (2022).
IL-33 induces NF-$\kappa$B activation in ILC2 that can be suppressed by in vivo and ex vivo 17$\beta$-estradiol. Frontiers in allergy 2022
Abstract
Asthmatic women tend to develop severe airway disease in their reproductive years, and 30%-40% of asthmatic women have peri-menstrual worsening of asthma symptoms. This indicates that fluctuations in ovarian hormones are involved in advancement of asthmatic disease and exacerbation of symptoms. Group 2 innate lymphoid cells, or ILC2, are readily detected in allergic conditions, such as rhinosinusitis, in individuals that develop nasal polyps do to allergen exposures, and in allergic asthma. ILC2 are airway localized immune cells activated by IL-33, an innate cytokine that perpetuates allergic inflammation by driving the production of IL-5 and IL-13. We have previously shown that ILC2 are highly activated in na{\{i}}ve and ovalbumin (OVA) challenged female BALB/c mice in comparison to male mice following stimulation with IL-33. Here we investigated the effect of steady-state ovarian hormones on ILC2 and the NF-$\kappa$B signaling pathway following OVA sensitization and challenge. We found that estrogen-treated ovariectomized mice (OVX-E2) that had been challenged with OVA had reduced IL-5 and IL-13 production by lung ILC2 as compared to lung ILC2 isolated from intact male and female sham-operated controls that had been treated with OVA. ILC2 were isolated from untreated animals and co-cultured ex vivo with and without estrogen plus IL-33. Those estrogen-treated ILC2 similarly produced less IL-5 and IL-13 in comparison to untreated and had reduced NF-$\kappa$B activation. Single-cell RNA sequencing showed that 120 genes were differentially expressed in male and female ILC2 and Nfkb1 was found among top-ranked regulatory interactions. Together these results provide new insight into the suppressive effect of estrogen on ILC2 which may be protective in female asthmatics. Understanding further how estrogen modulates ILC2 may provide therapeutic targets for the treatment of allergic diseases."
更多信息
| 种属 | Mouse |
|---|---|
| Magnet Compatibility | EasySep™ Magnet (Catalog #18000) |
| 样本来源 | Other |
| Selection Method | Negative |
标记抗体
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抗小鼠CD11c抗体，克隆N418抗小鼠CD11c的仓鼠（亚美尼亚）单克隆IgG抗体
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抗小鼠CD19抗体，克隆ID3大鼠单克隆IgG2a抗体，抗小鼠CD19
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抗小鼠CD3e抗体，克隆145-2C11亚美尼亚仓鼠单克隆IgG1抗体，抗小鼠CD3e
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抗小鼠CD45抗体，克隆30-F11大鼠单克隆IgG2b抗体，抗小鼠CD45
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抗小鼠Gr-1抗体，克隆RB6-8C5大鼠单克隆IgG2b抗体，抗小鼠Gr-1 （Ly-6G/Ly-6C）
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抗小鼠NK1.1（CD161）抗体，克隆PK136抗小鼠 NK1.1 (CD161) 的 (C3H x BALB/c) F1 杂交单克隆IgG2a抗体
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抗小鼠TCR Gamma/Delta 抗体，克隆GL3亚美尼亚仓鼠单克隆IgG2抗体，抗小鼠T细胞受体 gamma/delta
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抗小鼠TER119抗体，克隆TER-119抗小鼠TER119的大鼠（WI）单克隆IgG2b抗体
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抗小鼠CD11b抗体，克隆M1/70抗人、小鼠、恒河猴CD11b的大鼠单克隆IgG2b抗体
